Isolating Lysosomes from Rat Liver.
نویسنده
چکیده
This protocol describes the generation of a fraction enriched in lysosomes from rat liver. The lysosomes are rapidly isolated using density-gradient centrifugation with gradient media that retain the osmolarity of the lysosomes such that they are functional and can be used in in vitro assays.
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عنوان ژورنال:
- Cold Spring Harbor protocols
دوره 2016 4 شماره
صفحات -
تاریخ انتشار 2016